The calciferol receptor (vdr) is a nuclear hormone-binding transcription factor that manages the expression of genes interested in calcium and phosphate homeostasis, bone development, immune function, and cellular cycle control. VDR was originally seen in tissues included in calcium control and eventually was present in noncalcium-regulating tissues such as dermal fibroblasts and keratinocytes of the skin, immune cells, selected cardiovascular system cell types, and cell components of various organs (21).
Recent genome-wide studies employing ChIP deep sequencing have shown the VDR binds to distal cis components located numerous base pairs away from the regulated gene, thereby influencing the functional consequence of a signaling pathway rather than directly modulating a particular gene product. The identification these VDR reactive elements has resulted in the realization that vdr functions may be more widespread than initially thought, revealing added layers of complexity in gene regulation.
Activation of Vdr signaling inside the early erythroid progenitor citizenry of mouse button bone marrow by the calcemic agent calcitriol promotes expansion and delays maturation of erythroid precursors as discovered by clonogenic https://dataroomapps.net/data-management-made-simple-how-virtual-data-rooms-can-simplify-your-complex-business-processes assays, cell surface area phenotyping, and hematopoietic control cell marker analysis. These types of results illustrate that the vdr signaling path has a function in maintaining procreator potential and delay of erythroid differentiation, independent of its popular effects in calcium flux.
Activation from the Vdr signaling pathway by calcemic agent calcitriol also significantly boosts the number of BFU-E colonies formed in clonogenic assays in cKit+CD71lo/neg (early progenitors) vs cKit+CD71hi (late progenitors) populations of Linneg E12. 5 FLORIDA bone marrow cells. This increase in BFU-E colony numbers correlates with a decrease in methylation of the marketer region incorporating six hexameric VDR response elements.
